June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Retinal Expression of α-1-Antitrypsin (A1AT) in diabetic and non-diabetic rats
Author Affiliations & Notes
  • Gustavo Ortiz
    Ciencias Biomédicas, Universidad Austral, Pilar, Argentina
    Nanomedicine & Vision Group, Universidad Austral, Pilar, Argentina
  • Jorge Mancini
    Ciencias Biomédicas, Universidad Austral, Pilar, Argentina
    Nanomedicine & Vision Group, Universidad Austral, Pilar, Argentina
  • Eduardo Chuluyan
    Farmacología, Universidad de Buenos Aires, Buenos Aires, Argentina
  • Juan Gallo
    Ciencias Biomédicas, Universidad Austral, Pilar, Argentina
    Nanomedicine & Vision Group, Universidad Austral, Pilar, Argentina
  • Footnotes
    Commercial Relationships Gustavo Ortiz, None; Jorge Mancini, None; Eduardo Chuluyan, None; Juan Gallo, EP2186529 B1 (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6329. doi:
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      Gustavo Ortiz, Jorge Mancini, Eduardo Chuluyan, Juan Gallo, ; Retinal Expression of α-1-Antitrypsin (A1AT) in diabetic and non-diabetic rats. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6329.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Early stages of diabetic retinopathy are characterized by inflammatory changes. The role of several endogenous anti-inflammatory molecules in this disease is unknown. We aimed at evaluating the expression of A1AT in the retina of diabetic and non-diabetic rats.

Methods: Five male Wistar rats of 250 g were treated with an IP injection of 45mg/kg of streptozotocin (STZ). Animals with glycemia levels above 200 mg/dl were included in the study. Five non-diabetic rats were included as a control group. Animals were sacrificed after 16 weeks of diabetes. Cross sections of retinas were analyzed by immunohistochemistry using a primary antibody against A1AT. Protein expression was also analysed by western blot.

Results: Retinas of diabetic and control rats showed immunoreactivity of A1AT in the fiber layer, outer plexiform layer and in the external segment of photoreceptors. Higher staining was seen in diabetics. Small vessels were only stained among diabetic animals. No significant differences of protein expression of A1AT were observed between groups by westernblot.

Conclusions: An increased immunohistochemical staining of A1AT in diabetic rats and a similar protein expression between control and diabetic retinas might suggest a relative insufficient quantity of A1AT among diabetics after 16 weeks of disease. Studies in animals with longer duration of diabetes are being conducted to further evaluate the role of A1AT in diabetic retinopathy.

Keywords: 499 diabetic retinopathy • 557 inflammation • 688 retina  
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