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Hiroaki Takahashi, Tetsuo Kida, Seiko Kozai, Hideki Tokushige; Topical Bromfenac 0.1% suppresses Concanavalin-A Induced Blood Retinal Barrier Breakdown. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6363.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the inhibitory effect of topically applied bromfenac sodium (BF) 0.1% ophthalmic solution, a non-steroidal anti-inflammatory drug (NSAID) possessing potent cyclooxygenase (COX)-1 and COX-2 inhibitory activity, in rabbit with blood-retinal barrier (BRB) breakdown induced by intravitreally administered Concanavalin A (ConA), and also to compare the effect of BF 0.1% with other ophthalmic NSAIDs, nepafenac (NF) 0.1% and diclofenac (DF) sodium 0.1%, in this model.
Male Dutch-belted rabbits (2.0-2.5 kg) were used. ConA (5 μg/eye) or vehicle (sterile saline) was injected intravitreally to unilateral eye of the anesthetized rabbits. Rabbits were topically applied with 50 μL of BF 0.1%, NF 0.1% or DF 0.1% three times a day on days -1, 0, 1, 2 relative to ConA injection (day 0). Three days after ConA injection, rabbits were euthanized and the vitreous bodies were isolated. The protein concentration in the vitreous body was measured by BCA methods to assess BRB breakdown.
The vitreous protein concentrations in ConA -injected rabbits was 2117 ± 400 μg/mL (mean ± SD, n=6), and significantly increased compared with that in vehicle-injected rabbits (1307 ± 501 μg/mL (n=6)). The vitreous protein concentrations of BF 0.1%, NF 0.1%, and DF 0.1% groups were 1474 ± 574 (n=6), 1674 ± 452 (n=6), and 1800 ± 351 (n=7) μg/mL respectively. BF 0.1% significantly suppressed BRB breakdown in this model (p<0.05, Dunnett multiple comparisons test, one-side). However, NF 0.1% and DF 0.1% did not significantly suppress in this study.
Topical BF 0.1% was more potent than topical NF 01% and DF 0.1% in the ConA-induced BRB breakdown model. Therefore, it is suggested that topical BF 0.1% may have a therapeutical potential to retinal edema resulting from BRB breakdown induced by inflammation.
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