June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Fibronectin Adhesive Properties of Various Intraocular Lens Materials
Author Affiliations & Notes
  • Marcia Ong
    R&D, Alcon Research, Fort Worth, TX
  • Lixin Wang
    R&D, Alcon Research, Fort Worth, TX
  • Mutlu Karakelle
    R&D, Alcon Research, Fort Worth, TX
  • Footnotes
    Commercial Relationships Marcia Ong, Alcon Research (E); Lixin Wang, Alcon Laboratories Inc (E); Mutlu Karakelle, Novartis Alcon Division (E)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 819. doi:
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    • Get Citation

      Marcia Ong, Lixin Wang, Mutlu Karakelle; Fibronectin Adhesive Properties of Various Intraocular Lens Materials. Invest. Ophthalmol. Vis. Sci. 2013;54(15):819.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Decreased PCO rates are associated with hydrophobic acrylic IOLs. In addition to their square edge geometry, increased bioadhesive properties of the IOL material may also play a role contributing to the lower PCO rates. In this study, an in vitro fibronectin protein adhesion assay was performed to compare the amount of fibronectin adsorbed to various hydrophobic acrylic (Acrysof, HOYA and Tecnis) and polymethylmethacrylate (PMMA) IOLs.

Methods: Four different types of IOLs (Acrysof SN60WF, HOYA AF-1 iMicS 1 NY-60, Tecnis ZCB00, PMMA SK60CM) were used in the assay. A total of N=12 IOLs from each group were incubated with soluble fibronectin in Tris-buffered BSS (TBSS) at 37°C for 24 hrs. Another set of IOLs (N=12 per group) were also incubated with soluble fibronectin in Tris-buffered BSS (TBSS) at 37°C for 24 hrs followed by treatment with 2% sodium dodecyl sulfate (SDS) at 37°C for 30 minutes. The IOLs were then rinsed with BSS to remove unbound protein. The amount of fibronectin (μg) adhered to each IOL was quantified using the Micro BCA™ Protein Assay Kit (Thermo Scientific).

Results: Significantly more fibronectin was adsorbed to AcrySof and HOYA compared with Tecnis and PMMA (P<0.001), with no significant differences between Acrysof and HOYA (P=0.165) and Tecnis and PMMA (P=0.31). After SDS treatment, persistent fibronectin adsorption was the highest for AcrySof, followed by HOYA, Tecnis, then PMMA (P<0.001). Fibronectin retention was the highest for AcrySof (93.8%), followed by Tecnis (83%), HOYA (82.2%) then PMMA (69.2%), with significant differences between AcrySof and PMMA (P=0.011).

Conclusions: AcrySof IOL material has higher fibronectin bioadhesion properties compared with other hydrophobic acrylic and PMMA IOL materials. The higher fibronectin retention observed for AcrySof after treatment with an anionic detergent (SDS) suggests the presence of stronger bonds/interaction between the hydrophobic acrylic material surface and the fibronectin protein structure.

Keywords: 567 intraocular lens • 652 posterior capsular opacification (PCO)  

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