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Eleonora Favuzza, Iacopo Paladini, Ugo Menchini, Rita Mencucci; Square-Edge IOLs and Epithelial Lens Cell Proliferation: Implication on Posterior Capsule Opacification in an in Vitro Model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):852.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate human Lens Epithelial Cell (hLEC) proliferation comparing a 360-degree square-edge one-piece Intraocular Lens (IOL) (TECNIS ONE Abbott Medical Optics) to a square-edge three-piece IOL (AR40E, AMO) using an in vitro culture model
An in vitro Posterior Capsule Opacification (PCO) model was established: hLECs (104 from ATCC, USA) were seeded on transwell cell culture inserts coated with a type I collagen membrane on which an IOL (TECNIS ONE or AR40E) had been previously placed. As control, cells were plated on the insert membrane without an IOL and cultured at the same conditions. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated
At day six the cell number in inserts containing IOLs was significantly (P<0.05) lower than in controls. The inserts with TECNIS ONE contained a similar number of cells to AR40e (P>0.05 ); all the cells in each insert were viable. Cell density in the area under each IOL was significantly lower either than the cell density outside the IOL or in the control insert. Cell density under TECNIS ONE was lower but not significantly different from that under AR40E (P>0.05).
A 360-degree square edge is a key factor in PCO formation. Probably also an haptic design able to increase IOL capsular bag fixation is important in preventing PCO. Long term clinical evaluation is necessary to estimate functional results.
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