June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Reproducibility of SD-OCT-Based Ganglion-Cell-Complex-Layer Thickness in Early Glaucoma using Commercial and Custom Segmentation Algorithms
Author Affiliations & Notes
  • Mona Garvin
    Center for the Prevention and Treatment of Visual Loss, Iowa City VA Health Care System, Iowa City, IA
    Electrical and Computer Engineering, The University of Iowa, Iowa City, IA
  • Kyungmoo Lee
    Electrical and Computer Engineering, The University of Iowa, Iowa City, IA
  • Trudy Burns
    Epidemiology, The University of Iowa, Iowa City, IA
  • Michael Abramoff
    Center for the Prevention and Treatment of Visual Loss, Iowa City VA Health Care System, Iowa City, IA
    Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA
  • Andreas Wahle
    Center for the Prevention and Treatment of Visual Loss, Iowa City VA Health Care System, Iowa City, IA
    Electrical and Computer Engineering, The University of Iowa, Iowa City, IA
  • Milan Sonka
    Electrical and Computer Engineering, The University of Iowa, Iowa City, IA
    Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA
  • Young Kwon
    Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA
  • Footnotes
    Commercial Relationships Mona Garvin, Patent application 12/001,066 (P); Kyungmoo Lee, None; Trudy Burns, None; Michael Abramoff, IDx LLC (E), IDx LLC (I), University of Iowa (P); Andreas Wahle, None; Milan Sonka, US 7,995,810 (P); Young Kwon, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 88. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Mona Garvin, Kyungmoo Lee, Trudy Burns, Michael Abramoff, Andreas Wahle, Milan Sonka, Young Kwon; Reproducibility of SD-OCT-Based Ganglion-Cell-Complex-Layer Thickness in Early Glaucoma using Commercial and Custom Segmentation Algorithms. Invest. Ophthalmol. Vis. Sci. 2013;54(15):88.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

To compute the reproducibility of SD-OCT-based ganglion-cell-layer plus inner-plexiform-layer (GCL+IPL) thickness measurements from both a commercially available algorithm (Cirrus, Carl Zeiss Meditec, Inc.) and a custom-developed algorithm (from the suite of Iowa Reference Algorithms) in early glaucoma patients.

 
Methods
 

Macula SD-OCT volumes (Cirrus, Carl Zeiss Meditec, Inc., Dublin, CA, 200 x 200 x 1024 voxels, 6 x 6 x 2 mm3) were obtained prospectively in both eyes of 58 patients with open-angle glaucoma or glaucoma suspicion on two separate visits. The visits were at most four months apart. The combined GCL+IPL thickness was computed for each SD-OCT volume within an elliptical annulus centered at the fovea (with a vertical inner and outer radius of 0.5 mm and 2.0 mm, respectively; and horizontal inner and outer radius of 0.6 mm and 2.4 mm, respectively) based on two algorithms: (1) a previously published graph-theoretic layer segmentation approach developed at The University of Iowa and (2) the Ganglion Cell Analysis module of version 6 of the Zeiss Cirrus software. The mean overall thickness in the elliptical annulus was computed as well as the thickness within six sectors (Figure 1). For statistical analysis, the SD-OCT volumes of twelve eyes were excluded because of a low signal strength (≤ 5), image acquisition errors, or errors in performing the commercial GCL+IPL analysis in at least one of the repeat acquisitions.

 
Results
 

From the 104 analyzed eyes with repeated measurements, the intraclass correlation coefficient (ICC) for the overall elliptical annular GCL+IPL thickness was 0.98 (95% CI 0.97, 0.99) using the Iowa algorithm and 0.95 (0.93, 0.97) using the Cirrus algorithm; the intervisit standard deviation (SD) was 1.63 μm (Iowa) and 2.52 μm (Cirrus); and the coefficient of variation (CV) was 2.3% (Iowa) and 3.6% (Cirrus). The sector-based ICCs, intervisit SDs, and CVs are reported in Table 1. The ICCs were significantly higher using the Iowa approach except for Region S where there was no difference in the reproducibility.

 
Conclusions
 

SD-OCT-based ganglion cell thickness measurements in early glaucoma patients are highly reproducible.

 
 
Figure 1. (A) Central B-scan from example volume and (B) corresponding Iowa layer segmentation. (C) Example SD-OCT projection image and (D) six sectors for regional GCL+IPL analysis.
 
Figure 1. (A) Central B-scan from example volume and (B) corresponding Iowa layer segmentation. (C) Example SD-OCT projection image and (D) six sectors for regional GCL+IPL analysis.
   
Keywords: 550 imaging/image analysis: clinical • 531 ganglion cells • 629 optic nerve  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×