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Zhengri Li, Jung-Han Choi, Hyo Seok Lee, Ji-Suk Choi, Han Jin Oh, Kyung Chul Yoon; Effects of Omega-3 Fatty Acid and Hyaluronic acid Mixture Eye Drops on the Ocular Surface in Desiccating Stress induced Murine Dry Eye. Invest. Ophthalmol. Vis. Sci. 2013;54(15):901.
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To investigate the effects of topical application of omega-3 Fatty Acid (FA) and hyaluronic acid (HA) mixtures on inflammation and oxidative stress markers in a mouse model of experimental dry eye (EDE).
Eye drops consisting of 0.02%, or 0.2% omega-3 FA and 0.1% HA mixtures, or 0.1% HA were applied in desiccating stress induced murine dry eye. Corneal irregularity and corneal fluorescein staining scores were measured at 5 and 10 days after treatment. Levels of interleukin (IL)-1β, IL-17, and interferon gamma-induced protein (IP)-10 were measured in the conjunctiva using a multiplex immunobead assay at 10 days. The concentrations of HEL and 4-HNE were measured with enzyme-linked immunosorbent assays in conjunctiva tissue.
Although all parameters were lower in the 0.02% FA mixture and HA-treated groups compared with the EDE group, the effect was not statistically significant. However, mice treated with 0.2% omega-3 FA mixture showed a significant improvement in corneal irregularity and corneal fluorescein staining compared with EDE and HA-treated mice. A significant decrease in the levels of IL-1β, IL-17, and IP-10 were observed in the 0.2% FA mixture-treated group, compared with the other groups. In the 0.2% FA mixture-treated groups, the concentrations of HEL and 4-HNE were also lower than the other groups.
Topical omega-3 FA mixture eye drops can improve corneal irregularity and corneal epithelial barrier disruption, and decrease inflammatory cytokines and oxidative stress markers on the ocular surface. These results suggest that mixture of 0.2% Omega-3 FA and HA can be useful for treatment of dry eye treatment.
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