June 1975
Volume 14, Issue 6
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Articles  |   June 1975
Excitation and emission spectra of fluorescein dye in the human ocular fundus.
Investigative Ophthalmology & Visual Science June 1975, Vol.14, 487-492. doi:
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      F C Delori, I Ben-Sira; Excitation and emission spectra of fluorescein dye in the human ocular fundus.. Invest. Ophthalmol. Vis. Sci. 1975;14(6):487-492.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The excitation and emission spectra of fluorescein dye were determined during angiography from different sites in the human ocular fundus. All spectra were markedly shifted toward longer wavelengths relative to the spectra of fluorescein in aqueous solution. This effect is most pronounced for the macular area; however, it decreases for the choroidal background and even more for the retinal vessels. The results are relevant to the selection of optical filter combinations for fluorescein angiography.

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