August 1977
Volume 16, Issue 8
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Articles  |   August 1977
In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas.
Investigative Ophthalmology & Visual Science August 1977, Vol.16, 743-751. doi:
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      B E McCarey; In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas.. Invest. Ophthalmol. Vis. Sci. 1977;16(8):743-751.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Human donor corneas were stored in McCarey-Kaufman (M-K) medium for 3 to 6 days; then they were mounted in the specular microscope, and the endothelial surface perfused with a glutathione-bicarbonate-Ringer solution. During storage, the corneal thickness increased 37% above an assumed normal of 0.520 mm. The corneas did not have sufficient active thinning to be accepted as viable when tested by the temperature-reversal phenomenon. Since comparable donor corneas have been used in successful penetrating keratoplasties, there must be a discrepancy between the cell viability as tested by the temperature-reversal phenomenon and clinical application.

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