May 1979
Volume 18, Issue 5
Free
Articles  |   May 1979
A new technique for isolation of Descemet's membrane: preliminary studies.
Investigative Ophthalmology & Visual Science May 1979, Vol.18, 527-532. doi:
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      M C Kenney, P D Benya, M E Nimni, R E Smith; A new technique for isolation of Descemet's membrane: preliminary studies.. Invest. Ophthalmol. Vis. Sci. 1979;18(5):527-532.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The structure and function of basement membranes have been the subject of extensive investigation. The present study takes advantages of a new experimental procedure to yield ultrastructurally pure basement membranes and applies this methodology to Descemet's membrane, a highly specialized ocular basement membrane. Rabbit Descemet's membranes and associated endothelial cells were mechanically isolated without contaminating stromal elements. The endothelial cells were then solubilized and removed by treatment with detergents as verified by light microscopy and scanning and transmission electron microscopy. The Descemet's membrane remained intact and retained its fibrillar fine structure. Therefore purity of starting material for ongoing morphological and biochemical studies of isolated Descemet's membranes is demonstrated. These investigations will provide a valuable data base for comparison with disease-altered Descemet's membranes.

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