November 1987
Volume 28, Issue 11
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Articles  |   November 1987
Growth of retinal capillary endothelia using pericyte conditioned medium.
Author Affiliations
  • H C Wong
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, England.
  • M Boulton
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, England.
  • J Marshall
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, England.
  • P Clark
    Department of Clinical Ophthalmology, Institute of Ophthalmology, London, England.
Investigative Ophthalmology & Visual Science November 1987, Vol.28, 1767-1775. doi:
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      H C Wong, M Boulton, J Marshall, P Clark; Growth of retinal capillary endothelia using pericyte conditioned medium.. Invest. Ophthalmol. Vis. Sci. 1987;28(11):1767-1775.

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Abstract

Long-term cultures of bovine retinal capillary endothelial cells (BRCEC) have been established using bovine retinal pericyte conditioned medium (PCM). PCM had greater mitogenic activity on BRCEC than unconditioned medium (P less than 0.01). The mitogenic activity of PCM was not cell specific since bovine Tenon's fibroblast conditioned medium also had significant activity. PCM's activity was not enhanced by heparin though it was additive to that of retinal crude extract.

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