November 1987
Volume 28, Issue 11
Free
Articles  |   November 1987
Measurement of the volume flow and hydraulic conductivity across the isolated dog retinal pigment epithelium.
Author Affiliations
  • S Tsuboi
    Department of Ophthalmology, University of Minnesota, Minneapolis 55455.
Investigative Ophthalmology & Visual Science November 1987, Vol.28, 1776-1782. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      S Tsuboi; Measurement of the volume flow and hydraulic conductivity across the isolated dog retinal pigment epithelium.. Invest. Ophthalmol. Vis. Sci. 1987;28(11):1776-1782.

      Download citation file:


      © 2017 Association for Research in Vision and Ophthalmology.

      ×
  • Supplements
Abstract

The isolated dog retinal pigment epithelium (RPE)-choroid was gently stretched on the inner surface of a spherical stainless mesh, retinal side upward, and clamped between half-chambers made of Kel-F. The volume flow across the tissue was monitored by the movement of water in capillary tubes connected to both chambers. With zero pressure difference across the RPE-choroid, retina-to-choroid fluid flow was determined to be 6.4 microliters/hr/cm2 (absorption). Removal of HCO-3 from the solution did not affect the fluid flow. However, the flow was reduced 88% in Cl- -free medium, indicating a coupling between water and Cl- absorption. The flow was also inhibited by ouabain (10(-5) M) and furosemide (10(-4) M). Hydraulic conductivity (Lp) of the RPE-choroid was determined to be 0.0126 microliters/min/cm2/mm Hg which places the dog RPE-choroid in the category of a "leaky" epithelium.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×