July 1987
Volume 28, Issue 7
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Articles  |   July 1987
Nomarski evaluation of rod outer segment renewal in a hereditary retinal degeneration. Comparison with autoradiographic evaluation.
Investigative Ophthalmology & Visual Science July 1987, Vol.28, 1049-1058. doi:
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      G Aguirre, L Andrews; Nomarski evaluation of rod outer segment renewal in a hereditary retinal degeneration. Comparison with autoradiographic evaluation.. Invest. Ophthalmol. Vis. Sci. 1987;28(7):1049-1058.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Differential interference contrast (Nomarski) optics and autoradiography were used to estimate independently the rate of outer segment renewal in the early stages of progressive rod-cone degeneration (PRCD) in dogs. We have found that significant reductions in renewal rate are demonstrable using the two methods of analysis: autoradiography = 34% reduction; Nomarski method = 18% reduction. Nomarski analysis of affected rod outer segments (ROS) demonstrated optical periodicities (light/dark bands) extending from the base to the tip of the outer segment. Nineteen percent of the cells, however, showed areas where the light/dark bands were not apparent. These homogeneous, "optically silent" regions were interpreted to possibly represent areas where the disc orientation was not perpendicular to the long axis of the ROS.

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