July 1987
Volume 28, Issue 7
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Articles  |   July 1987
Non-tryptophan fluorescence of crystallins from normal and cataractous human lenses.
Investigative Ophthalmology & Visual Science July 1987, Vol.28, 1157-1163. doi:
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      G J Bessems, E Keizer, J Wollensak, H J Hoenders; Non-tryptophan fluorescence of crystallins from normal and cataractous human lenses.. Invest. Ophthalmol. Vis. Sci. 1987;28(7):1157-1163.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The soluble proteins of a newborn human lens exhibit almost no non-tryptophan fluorescence. On aging, an increase in the fluorescence of all native crystallins is found except for gamma 2-crystallin. With formation of nuclear cataract, a further increase is seen for gamma 1-crystallin. The fluorophore, excitation 355 nm/emission 420 nm, is mainly associated with one species of the gamma 1-crystallin population. It is also present as such in the soluble fraction and increases significantly with nuclear cataract formation. At least one of the gamma 1-crystallins seems to play an important role in the cataractogenic process.

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