August 1991
Volume 32, Issue 9
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Articles  |   August 1991
The influence of 12(R)-hydroxyeicosatetraenoic acid on ciliary epithelial sodium, potassium-adenosine triphosphatase activity and intraocular pressure in the rabbit.
Author Affiliations
  • N A Delamere
    Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, Louisville 40292.
  • R R Socci
    Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, Louisville 40292.
  • K L King
    Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, Louisville 40292.
  • P Bhattacherjee
    Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, Louisville 40292.
Investigative Ophthalmology & Visual Science August 1991, Vol.32, 2511-2514. doi:
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      N A Delamere, R R Socci, K L King, P Bhattacherjee; The influence of 12(R)-hydroxyeicosatetraenoic acid on ciliary epithelial sodium, potassium-adenosine triphosphatase activity and intraocular pressure in the rabbit.. Invest. Ophthalmol. Vis. Sci. 1991;32(9):2511-2514.

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Abstract

Ciliary epithelium was isolated from the rabbit eye and used as a source of plasma membrane material for sodium, potassium-adenosine triphosphatase (Na,K-ATPase) measurements. In the presence of 12(R)-hydroxyeicosatetraenoic acid [12(R)HETE], Na,K-ATPase (ouabain-sensitive ATPase) activity was reduced from 22.5 to 16.3 microM phosphate released/mg protein/hr. Ouabain-insensitive ATPase activity was not altered by 12(R)HETE. No changes in ciliary epithelium ATPase activity were observed in the presence of 12(S)HETE. In parallel studies with conscious rabbits, 12(R)HETE applied topically to the eye was found to lower intraocular pressure (IOP). It is possible that the IOP-lowering effect of 12(R)HETE may be, in part, associated with its ability to suppress the Na,K-ATPase activity of the ciliary epithelium.

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