July 1991
Volume 32, Issue 8
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Articles  |   July 1991
Class II antigen expression by lacrimal epithelial cells. An updated working hypothesis for antigen presentation by epithelial cells.
Author Affiliations
  • A K Mircheff
    Department of Physiology & Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
  • J P Gierow
    Department of Physiology & Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
  • L M Lee
    Department of Physiology & Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
  • R W Lambert
    Department of Physiology & Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
  • R H Akashi
    Department of Physiology & Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
  • F M Hofman
    Department of Physiology & Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
Investigative Ophthalmology & Visual Science July 1991, Vol.32, 2302-2310. doi:
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    • Get Citation

      A K Mircheff, J P Gierow, L M Lee, R W Lambert, R H Akashi, F M Hofman; Class II antigen expression by lacrimal epithelial cells. An updated working hypothesis for antigen presentation by epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1991;32(8):2302-2310.

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Abstract

It has been suggested that aberrant expression of Class II histocompatibility antigens (HLA) is involved in T cell activation and leads to autoimmunity. Although Class II antigen expression was found in various nonlymphoid tissues, including salivary glands, its expression on lacrimal epithelial cells has not been reported. In this study, 12 cadaver lacrimal glands were analyzed for HLA-DR and for the numbers and distributions of T suppressor cells (Ts), T helper cells (Th), B cells, and macrophages. None of these cases exhibited the high numbers of inflammatory cells, tissue damage, and fibrosis characteristic of Sjogren's syndrome. The HLA-DR-positive epithelial cells were detected in ten cases; they represented from less than 1% to more than 70% of the epithelial cells. In these ten positive cases, there were greater numbers of T cells per millimeter squared (229 +/- 94 [mean +/- the standard error of the mean]) than in the two HLA-DR-negative cases (37 +/- 1 [mean +/- range]). Three lacrimal gland specimens tested were negative for immunoglobulin (Ig) G-bearing B cells, and two of the three specimens tested had IgA-bearing cells. Acinar cells were isolated from rat and rabbit lacrimal glands and cultured overnight in serum-free media supplemented with several potential mediators of Class II antigen expression: interferon-gamma, carbachol, or isoproterenol. Freshly isolated cells did not express Class II antigens at detectable levels, but in most experiments, they began to express the antigen even in the absence of putative mediators.(ABSTRACT TRUNCATED AT 250 WORDS)

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