December 1991
Volume 32, Issue 13
Free
Articles  |   December 1991
Transplanted photoreceptors identified in dystrophic mouse retina by a transgenic reporter gene.
Author Affiliations
  • P Gouras
    Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
  • J Du
    Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
  • H Kjeldbye
    Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
  • R Kwun
    Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
  • R Lopez
    Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
  • D J Zack
    Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
Investigative Ophthalmology & Visual Science December 1991, Vol.32, 3167-3174. doi:
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    • Get Citation

      P Gouras, J Du, H Kjeldbye, R Kwun, R Lopez, D J Zack; Transplanted photoreceptors identified in dystrophic mouse retina by a transgenic reporter gene.. Invest. Ophthalmol. Vis. Sci. 1991;32(13):3167-3174.

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Abstract

Dissociated photoreceptor cells from a transgenic strain of mice, containing a bovine promoter lac Z gene construct, were transplanted to the dystrophic retinas of a strain of mice (C3H, rd/rd) without obvious photoreceptors. The transgenic photoreceptor cells expressed beta-galactosidase and were distinguishable from the host retinal cells by light and electron microscopy after the 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside histochemical reaction. These results showed that transplanted transgenic photoreceptor cells survived at least 1 month in the host retina and had a primitive outer segment and a well-developed synaptic terminal.

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