February 1991
Volume 32, Issue 2
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Articles  |   February 1991
A reproducible method for injecting the mouse corneal stroma.
Author Affiliations
  • R L Hendricks
    Department of Ophthalmology, University of Illinois, Chicago 60612.
  • R J Epstein
    Department of Ophthalmology, University of Illinois, Chicago 60612.
  • M A Viana
    Department of Ophthalmology, University of Illinois, Chicago 60612.
  • D A Hoffmann
    Department of Ophthalmology, University of Illinois, Chicago 60612.
Investigative Ophthalmology & Visual Science February 1991, Vol.32, 366-370. doi:
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      R L Hendricks, R J Epstein, M A Viana, D A Hoffmann; A reproducible method for injecting the mouse corneal stroma.. Invest. Ophthalmol. Vis. Sci. 1991;32(2):366-370.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

An improved delivery system for injecting the mouse corneal stroma was developed. This system incorporates the following features: a repeating dispenser that eliminates inaccuracies in depressing a syringe plunger, foot activation which frees both hands for manipulating the needle and permits constant observation of the injection site, and a flexible 30-cm, 32-gauge stainless steel needle with a 30 degrees bevel and a locking hub that resists pulsation due to back pressure while permitting freedom of motion by the operator. These injections were done while observing the cornea with a vertically mounted slit lamp, ideally suited for examining and photographing the eyes of laboratory animals. The reproducibility of the new delivery system, expressed in terms of the signal-to-noise ratio, was estimated and compared with that of a hand-held microsyringe by injecting a solution of radioactive chromium into the corneal stroma of A/J mice. The eyes were removed within 1 hr of injection, and the amount of chromium in each eye was determined in a gamma counter. The new delivery system had significantly (P less than 0.05) greater reproducibility than the hand-held syringe and could be calibrated to deliver up to 0.65 microliter to the mouse cornea.

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