February 1991
Volume 32, Issue 2
Free
Articles  |   February 1991
Flow cytometry measurements of the DNA content of corneal epithelial cells during wound healing.
Author Affiliations
  • H W Thompson
    Louisiana State University Medical Center School of Medicine, New Orleans 70112.
  • J S Malter
    Louisiana State University Medical Center School of Medicine, New Orleans 70112.
  • T L Steinemann
    Louisiana State University Medical Center School of Medicine, New Orleans 70112.
  • R W Beuerman
    Louisiana State University Medical Center School of Medicine, New Orleans 70112.
Investigative Ophthalmology & Visual Science February 1991, Vol.32, 433-436. doi:
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    • Get Citation

      H W Thompson, J S Malter, T L Steinemann, R W Beuerman; Flow cytometry measurements of the DNA content of corneal epithelial cells during wound healing.. Invest. Ophthalmol. Vis. Sci. 1991;32(2):433-436.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

This study presents the first application of flow cytometry (FCM) techniques to the assessment of cell cycle dynamics in the corneal epithelium after experimental wounding. Anterior keratectomies 6 mm in diameter were created in the central corneas of albino rabbits. The authors sampled the epithelial tissue obtained outside the wound at 12-hr intervals until wound closure at 72 hr. Regenerated epithelium from the surface of the wounded area was collected at 78 hr. The percentages of nuclei in the G0/G1 (growth), S (DNA synthesis), and G2/M (tetraploid/mitosis) phases were determined by FCM. An increase in the percentage of nuclei in the G2/M phase at 36 hr was seen, compared with cell populations in samples from unwounded control corneas. The authors found an increase in mitotic activity in the corneal epithelium during the period of cell migration before wound closure.

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