March 1991
Volume 32, Issue 3
Free
Articles  |   March 1991
Human crystalline lens phospholipid analysis with age.
Author Affiliations
  • T E Merchant
    Pathologisch Instituut, Rijksuniversiteit Utrecht, The Netherlands.
  • J H Lass
    Pathologisch Instituut, Rijksuniversiteit Utrecht, The Netherlands.
  • P Meneses
    Pathologisch Instituut, Rijksuniversiteit Utrecht, The Netherlands.
  • J V Greiner
    Pathologisch Instituut, Rijksuniversiteit Utrecht, The Netherlands.
  • T Glonek
    Pathologisch Instituut, Rijksuniversiteit Utrecht, The Netherlands.
Investigative Ophthalmology & Visual Science March 1991, Vol.32, 549-555. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T E Merchant, J H Lass, P Meneses, J V Greiner, T Glonek; Human crystalline lens phospholipid analysis with age.. Invest. Ophthalmol. Vis. Sci. 1991;32(3):549-555.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Paired human crystalline lenses (n = 21, patient ages 20-79 years) were extracted for lipids with chloroform-methanol 2:1, using the Folch method. The extracted crude lipids were analyzed at 202.4 MHz by phosphorus-31 magnetic resonance spectroscopy (31P NMR). Fourteen membrane phospholipids were detected including phosphatidylcholine (PC), lysophosphatidylcholine (LPC), phosphatidylcholine plasmalogen (PC plas), phosphatidylethanolamine (PE), phosphatidylethanolamine plasmalogen, lysophosphatidylethanolamine (PA), phosphatidylglycerol (PG), lysophosphatidylglycerol, phosphatidylserine (PS), phosphatidic acid, phosphatidylinositol, sphingomyelin (SPH), and two uncharacterized phospholipids. The uncharacterized phospholipid at 0.13 was the predominant phospholipid, comprising 43.20% of the lens phospholipid profile. A decrease in mole percent of phosphorus concentrations of PE, PC plas, and PC and an increase in SPH correlated with age. The following computed indices decreased with age: PC/PG and PE/PS; PC + PE; (PC + PC plas); and PC/PS. The following computed indices increased with age: (PC + SPH)/(PE + PS), SPH/PG, (PC + SPH)/(PE + PS), LPC/PC, LPE/PE, SPH/PE, and SPH/PC. Changes in membrane phospholipids of the crystalline lens with age as detected by 31P NMR can be used to fingerprint lens maturation.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×