July 1991
Volume 32, Issue 8
Free
Articles  |   July 1991
31P magnetic resonance spectroscopy (MRS) of experimental orbital myositis.
Author Affiliations
  • S Winguth
    Department of Ophthalmology, University of California, San Francisco.
  • J Kurhanewicz
    Department of Ophthalmology, University of California, San Francisco.
  • M L Wang
    Department of Ophthalmology, University of California, San Francisco.
  • J M Quivey
    Department of Ophthalmology, University of California, San Francisco.
  • T L James
    Department of Ophthalmology, University of California, San Francisco.
  • D H Char
    Department of Ophthalmology, University of California, San Francisco.
Investigative Ophthalmology & Visual Science July 1991, Vol.32, 2417-2422. doi:
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    • Get Citation

      S Winguth, J Kurhanewicz, M L Wang, J M Quivey, T L James, D H Char; 31P magnetic resonance spectroscopy (MRS) of experimental orbital myositis.. Invest. Ophthalmol. Vis. Sci. 1991;32(8):2417-2422.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

We described a model of orbital myositis that was induced by 12-0-tetradecanoyl-phorbol-13-acetate (TPA) injection into the superior rectus muscle of New Zealand white rabbits. In this study, in vivo 31P magnetic resonance spectroscopy (MRS) was performed with a 4.7 Tesla Oxford (Oxford Instruments, Oxford, England) magnet to monitor the evolution of muscle inflammation in control animals and the response of this model to external beam radiation. 31P MRS showed a dramatic increase in high-energy phosphorus and phospholipid metabolites 48 hr after TPA injection. These spectra were similar to those from implanted allogenic fibroblasts. Within 18-48 hr after a single dose (400 cGy) or sequential doses (3 days at 400 cGy) of orbital irradiation, reduced extraocular muscle swelling and a significant decrease of all 31P metabolites occurred. A decrease (63 +/- 6%) in the signal-to-noise (S/N) ratio of the control inflamed muscle 31P MR spectra increased by 28 days after inflammation. Two days after single-dose radiation, 31P MR metabolites were significantly lower (58 +/- 5%, P less than 0.012) than control spectra. These postradiation spectra mirror the 28-day control spectra and are consistent with previous histologic data that show decreased fibroblastic activity. Change in 31P MRS was a sensitive indicator of treatment response latency.

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