April 1994
Volume 35, Issue 5
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Articles  |   April 1994
Expression of phototransduction cascade genes in the ground squirrel retina.
Author Affiliations
  • M von Schantz
    Department of Zoology, University of Göteborg, Sweden.
  • A Szél
    Department of Zoology, University of Göteborg, Sweden.
  • T van Veen
    Department of Zoology, University of Göteborg, Sweden.
  • D B Farber
    Department of Zoology, University of Göteborg, Sweden.
Investigative Ophthalmology & Visual Science April 1994, Vol.35, 2558-2566. doi:
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    • Get Citation

      M von Schantz, A Szél, T van Veen, D B Farber; Expression of phototransduction cascade genes in the ground squirrel retina.. Invest. Ophthalmol. Vis. Sci. 1994;35(5):2558-2566.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: This study describes the expression and distribution of phototransduction cascade gene products in the cone-dominant retina of the ground squirrel Spermophilus tridecemlineatus. METHODS: Messenger RNA expression was studied by blot hybridization, and the distribution of the gene products was investigated by immunocytochemistry. RESULTS: RNA blot hybridization showed messages for the alpha 2, beta 1, and beta 3 subunits of transducin but was negative for rhodopsin, alpha 1-transducin, and the alpha, beta, and gamma subunits of cyclic guanosine monophosphate (cGMP) phosphodiesterase. Immunocytochemical labeling indicated that the approximate ratio of the photoreceptor types in ground squirrel retina is 90.6% for green cones, 6.3% for rod-like cells, and 3.1% for blue cones. Rod-like cells were immunopositive for rhodopsin and blue opsin. All photoreceptor elements were labeled by antibodies against alpha 1-transducin (which recognizes both the alpha 1 and alpha 2 isoforms), beta 3-transducin, and the rod gamma subunit of phosphodiesterase, whereas no cells were labeled by antibodies against the rod alpha and beta subunits of phosphodiesterase or against the rod cGMP-gated cation channel. Rod-like cells and blue cones were stained by antibodies against beta 1-transducin. CONCLUSIONS: The authors demonstrate new cone-like traits in the biochemical make-up of rod-like cells, and a distribution of the transducin beta subunit in the ground squirrel is different from that found in other mammals.

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