January 1994
Volume 35, Issue 1
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Articles  |   January 1994
Heterogeneity in ultrastructure and elemental composition of perinuclear lens retrodots.
Author Affiliations
  • G F Vrensen
    Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
  • B Willekens
    Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
  • P T De Jong
    Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
  • G A Shun-Shin
    Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
  • N P Brown
    Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
  • A J Bron
    Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
Investigative Ophthalmology & Visual Science January 1994, Vol.35, 199-206. doi:
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      G F Vrensen, B Willekens, P T De Jong, G A Shun-Shin, N P Brown, A J Bron; Heterogeneity in ultrastructure and elemental composition of perinuclear lens retrodots.. Invest. Ophthalmol. Vis. Sci. 1994;35(1):199-206.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To unravel the cataractogenic process(es) leading to the birefringent lenticular bodies known as perinuclear retrodots. METHODS: Ten human lenses containing biomicroscopically verified perinuclear retrodots were systematically screened and analyzed using scanning electron microscopy and energy dispersive x-ray microanalysis to verify their ultrastructure and elemental composition. RESULTS: Three types of retrodots were distinguished, different in size, ultrastructure, and origin. Two of them contained calcium phosphate, the third probably contained calcium oxalate. All three types were separated from surrounding normal fibers and the crystalline inclusions were sequestered within membrane-lined bodies. CONCLUSIONS: Because of these observations and data found in the literature it is postulated that elevated free calcium is the initiating factor in the formation of retrodots, trapped by either oxalate or phosphate and sequestered in the retrodots. It is suggested that the oxalate is derived from ascorbate because of impaired protection against oxidative stress in the older lens. Phosphoric acid is believed to be released by calcium-induced hydrolysis of membrane phospholipids.

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