February 1994
Volume 35, Issue 2
Free
Articles  |   February 1994
Linkage of photoreceptor degeneration by apoptosis with inherited defect in phototransduction.
Author Affiliations
  • R N Lolley
    Department of Anatomy and Cell Biology, Jules Stein Eye Institute, UCLA School of Medicine.
  • H Rong
    Department of Anatomy and Cell Biology, Jules Stein Eye Institute, UCLA School of Medicine.
  • C M Craft
    Department of Anatomy and Cell Biology, Jules Stein Eye Institute, UCLA School of Medicine.
Investigative Ophthalmology & Visual Science February 1994, Vol.35, 358-362. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R N Lolley, H Rong, C M Craft; Linkage of photoreceptor degeneration by apoptosis with inherited defect in phototransduction.. Invest. Ophthalmol. Vis. Sci. 1994;35(2):358-362.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To investigate the developing retina of normal and rd/rd mice to establish if the inherited defect in the retinal degeneration (rd) gene, encoding the beta subunit of the cascade phosphodiesterase, is associated with rd photoreceptor degeneration by apoptosis. METHODS: DNA content of developing normal and rd/rd retinas was measured spectrophotometrically and analyzed for differential loss during the course of photoreceptor degeneration. Degenerating rd photoreceptors were evaluated by electron microscopy for cytoplasmic features and chromosomal condensation. DNA fragmentation was analyzed by agarose gel electrophoresis at daily intervals during the developmental period in which rd/rd cell death occurs. RESULTS: DNA loss from developing rd/rd retinas is maximal between 10 and 15 postnatal days. Photoreceptor cells die individually throughout the postnatal period of degeneration, with pycnotic nuclei dispersed among morphologically normal rd photoreceptors. DNA fragmentation into 200 base pair multiples occurs maximally in rd/rd retinas between 10 and 15 postnatal days. CONCLUSION: Photoreceptor cell death in developing rd/rd retinas occurs by a mechanism that links a defect in the phototransduction cascade with a program for cell death, called apoptosis.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×