January 1995
Volume 36, Issue 1
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Articles  |   January 1995
Characterization of arachidonic acid metabolism and the polymorphonuclear leukocyte response in mice infected intracorneally with Pseudomonas aeruginosa.
Author Affiliations
  • K A Kernacki
    Department of Immunology/Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
  • R S Berk
    Department of Immunology/Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Investigative Ophthalmology & Visual Science January 1995, Vol.36, 16-23. doi:
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      K A Kernacki, R S Berk; Characterization of arachidonic acid metabolism and the polymorphonuclear leukocyte response in mice infected intracorneally with Pseudomonas aeruginosa.. Invest. Ophthalmol. Vis. Sci. 1995;36(1):16-23.

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Abstract

PURPOSE: To examine the activity of myeloperoxidase (MPO) and the concentrations of the proinflammatory metabolites of arachidonic acid (AA) in ocular tissue of mice that are either capable or incapable of restoring corneal clarity during an intraocular Pseudomonas aeruginosa infection. METHODS: For a period of 11 days after infection, whole eyes were enucleated and homogenized in buffer from mice given only an initial infection as well as from mice given a subsequent infection in the previously uninfected eye either 4 or 8 weeks after the initial infection. Tissue-free supernatants from the ocular homogenates were used for the determination of MPO activity by quantitating the conversion of specific substrate by spectrophotometric methods and for the quantitation of AA metabolites by ELISA: RESULTS: Overall, animals reinfected at 4 and 8 weeks had a lower inflammatory response when compared to the mice given only the initial infection. The lowest levels of LTB4 and MPO activity, indicators of PMN involvement, were observed in the the 8-week reinfected mice, which restored corneal clarity in an enhanced manner. CONCLUSIONS: These results suggest that induced ocular PMN responses may play a role, in part, in the inflammatory response leading to the tissue destruction observed during ocular P. aeruginosa infection.

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