April 1995
Volume 36, Issue 5
Free
Articles  |   April 1995
Retinal ganglion cell death in experimental glaucoma and after axotomy occurs by apoptosis.
Author Affiliations
  • H A Quigley
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
  • R W Nickells
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
  • L A Kerrigan
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
  • M E Pease
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
  • D J Thibault
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
  • D J Zack
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
Investigative Ophthalmology & Visual Science April 1995, Vol.36, 774-786. doi:
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    • Get Citation

      H A Quigley, R W Nickells, L A Kerrigan, M E Pease, D J Thibault, D J Zack; Retinal ganglion cell death in experimental glaucoma and after axotomy occurs by apoptosis.. Invest. Ophthalmol. Vis. Sci. 1995;36(5):774-786.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

PURPOSE: To investigate whether retinal ganglion cell death in experimental glaucoma and after axotomy occurs by apoptosis. METHODS: Chronic elevated eye pressure was produced in 20 monkey eyes, and the optic nerve was transected unilaterally in the orbit of 10 monkeys and 14 rabbits. Sixteen monkey and 14 rabbit eyes were studied as normal controls. Analytic methods included light and electron microscopy, histochemistry for DNA fragmentation (TUNEL method), and DNA electrophoresis in agarose gels. RESULTS: Dying ganglion cells in the experimental retinas exhibited morphologic features of apoptosis, including chromatin condensation and formation of apoptotic bodies. Cells with a positive reaction for DNA fragmentation were observed in eyes subjected to axotomy and experimental glaucoma but were only rarely encountered in control eyes. No evidence of internucleosomal fragmentation was detected electrophoretically, possibly because of the small proportion of cells that were dying at any given time. CONCLUSION: Some retinal ganglion cells injured by glaucoma and by axotomy die by apoptosis.

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