February 1994
Volume 35, Issue 2
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Articles  |   February 1994
TGF-beta 2 and TGF-beta 3 immunoreactivity within the ciliary epithelium [corrected]
Author Affiliations
  • N S Peress
    Department of Pathology, State University of New York at Stony Brook.
  • E Perillo
    Department of Pathology, State University of New York at Stony Brook.
Investigative Ophthalmology & Visual Science February 1994, Vol.35, 453-457. doi:
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    • Get Citation

      N S Peress, E Perillo; TGF-beta 2 and TGF-beta 3 immunoreactivity within the ciliary epithelium [corrected]. Invest. Ophthalmol. Vis. Sci. 1994;35(2):453-457.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine whether the ciliary epithelium exhibits immunoreactivity for antibodies to transforming growth factor beta (TGF-beta) 2 and TGF-beta 3. The hypothesis was that because the aqueous humor contains mainly biologically active TGF-beta 2, with little TGF-beta 1, the epithelium largely responsible for its composition would also contain this isoform of TGF-beta. The authors anticipated TGF-beta 3 immunoreactivity because TGF-beta 3 often co-localizes with TGF-beta 2. METHODS: The authors followed a standard immunohistochemical protocol using the avidin-biotin complex and newly available rabbit antibodies to synthetic peptide sequences of TGF-beta 2 and TGF-beta 3. Formalin-fixed, paraffin-embedded samples of freshly obtained rabbit and human autopsy eyes were studied. Specificity was supported by specific peptide absorption of antisera before tissue incubation. RESULTS: The pigmented and nonpigmented ciliary epithelia of rabbit and human eyes were stained by antibodies to both TGF-beta 2 and TGF beta-3, and the staining was inhibited by preabsorption of antibodies by peptides of TGF-beta 2 and TGF-beta 3. CONCLUSIONS: The authors conclude that the ciliary epithelium exhibits TGF-beta 2- and TGF-beta 3-like immunoreactivity that, based upon complementary work from other laboratories, is probably synthesized by this epithelium and is not simply absorbed by it from the aqueous humor.

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