January 1995
Volume 36, Issue 1
Free
Articles  |   January 1995
C-fos gene expression in rat retinal cells after focal retinal injury.
Author Affiliations
  • K Yoshida
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • Y Muraki
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • K Ohki
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • T Harada
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • T Ohashi
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • H Matsuda
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • J Imaki
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
Investigative Ophthalmology & Visual Science January 1995, Vol.36, 251-254. doi:
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    • Get Citation

      K Yoshida, Y Muraki, K Ohki, T Harada, T Ohashi, H Matsuda, J Imaki; C-fos gene expression in rat retinal cells after focal retinal injury.. Invest. Ophthalmol. Vis. Sci. 1995;36(1):251-254.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To examine the expression of c-fos proto-oncogene in the rat retina after focal retinal injury. METHODS: A penetrating wound was made by pushing a 22-gauge hypodermic needle through the retina into the vitreous. The retinas were analyzed by in situ hybridization using single-stranded RNA probes for c-fos transcripts and by immunocytochemistry using anti-S100 protein antibody. RESULTS: Thirty minutes after the penetrating wound, c-fos mRNA was expressed in the inner nuclear layer (INL) and the ganglion cell layer (GCL) of the retina. Immunocytochemistry before in situ hybridization demonstrated c-fos mRNA surrounding the S-100 protein immunoreactive cytoplasm in the middle layer of the INL. CONCLUSIONS: Focal retinal injury of the rat retina induced the expression of c-fos mRNA in retinal cells. In the INL, it is suggested that the major cells that express c-fos mRNA after focal retinal injury were the Müller cells. These results suggest that c-fos may be involved in the transcription in the Müller cells after injury to the retina.

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