April 1992
Volume 33, Issue 5
Free
Articles  |   April 1992
EGF, EGF receptor, basic FGF, TGF beta-1, and IL-1 alpha mRNA in human corneal epithelial cells and stromal fibroblasts.
Author Affiliations
  • S E Wilson
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
  • Y G He
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
  • S A Lloyd
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
Investigative Ophthalmology & Visual Science April 1992, Vol.33, 1756-1765. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      S E Wilson, Y G He, S A Lloyd; EGF, EGF receptor, basic FGF, TGF beta-1, and IL-1 alpha mRNA in human corneal epithelial cells and stromal fibroblasts.. Invest. Ophthalmol. Vis. Sci. 1992;33(5):1756-1765.

      Download citation file:


      © 2015 Association for Research in Vision and Ophthalmology.

      ×
  • Supplements

We sought to determine whether human corneal epithelial cells and stromal fibroblasts synthesize messenger RNAs (mRNA) coding for epidermal growth factor (EGF), EGF receptor, basic fibroblast growth factor (basic FGF), transforming growth factor-beta 1 (TGF-beta 1), and interleukin-1 alpha (IL-1 alpha). Total cellular RNA was extracted from cultured stromal fibroblasts and ex vivo and cultured corneal epithelial cells. Oligo dt-primed complementary DNA (cDNA) was synthesized from each RNA sample. The polymerase chain reaction (PCR) was used to amplify sequences for EGF, EGF receptor, basic FGF, TGF-beta 1, IL-1 alpha, and beta actin from cDNA samples from each cell type. Southern blots of the PCR products were probed with oligonucleotides complementary to internal sequences within each of the amplified products. The amplification products were shown to be specific. For each modulator, the amplification product of the expected size was identified with at least one specific, alternative amplification product. The alternative splicing products suggest that there may be alternative mRNA splicing for each of the modulators studied. Differences were noted in the IL-1 alpha specific amplification products in stromal fibroblasts compared to corneal epithelial cells. EGF and EGF receptor mRNA production in human corneal epithelial cells and stromal fibroblasts suggest an autocrine role for EGF in the physiology of each of these cell types.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×