November 1995
Volume 36, Issue 12
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Articles  |   November 1995
Photodynamic therapy of ocular melanoma with bis silicon 2,3-naphthalocyanine in a rabbit model.
Author Affiliations
  • R A Hill
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • S Reddi
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • M E Kenney
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • J Ryan
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • L H Liaw
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • J Garrett
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • J Shirk
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • G Cheng
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • T Krasieva
    Department of Ophthalmology, University of California, Irvine 92717, USA.
  • M W Berns
    Department of Ophthalmology, University of California, Irvine 92717, USA.
Investigative Ophthalmology & Visual Science November 1995, Vol.36, 2476-2481. doi:
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    • Get Citation

      R A Hill, S Reddi, M E Kenney, J Ryan, L H Liaw, J Garrett, J Shirk, G Cheng, T Krasieva, M W Berns; Photodynamic therapy of ocular melanoma with bis silicon 2,3-naphthalocyanine in a rabbit model.. Invest. Ophthalmol. Vis. Sci. 1995;36(12):2476-2481.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To investigate bis (tri-n-hexylsiloxy) silicon 2,3-naphthalocyanine (SINc; 0.5 mg/kg) for photodynamic therapy of an experimental ocular melanoma in pigmented rabbits. METHODS: SINc was dissolved in canola oil by heating, emulsified with Tween 80, and administered by ear vein. Pharmacokinetics were studied in frozen tumor sections by fluorescence microscopy using a charge coupled device, camera-based, low-light detection system with digital image processing at 1 and 24 hours. A Ti:sapphire laser and a microlens were used to deliver the light (770 nm; 40 mW/cm2; 20 J/cm2). A control rabbit received light without SINc. RESULTS: Localization studies of SINc showed intravascular distribution shifting to a tumor stromal and perivascular distribution 24 hours after treatment. Tissue thermal damage after irradiation was minimal in the control. Exudative retinal detachments were not observed. Tumor destruction was observed, with sharp demarcation to a depth of 3.5 mm. CONCLUSIONS: Tumor light penetration was good at 770 nm, and thermal effects from the exciting light alone were minimal. Photodynamic therapy with SINc resulted in localized tumor destruction reflecting the light beam path without damage to adjacent tissue or intraocular complications.

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