May 1995
Volume 36, Issue 6
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Articles  |   May 1995
Modification of vitamin E during ischemia-reperfusion in rat retina.
Author Affiliations
  • A M Bron
    Department of Ophthalmology, University of Burgundy, Dijon, France.
  • V Maupoil
    Department of Ophthalmology, University of Burgundy, Dijon, France.
  • C Garcher
    Department of Ophthalmology, University of Burgundy, Dijon, France.
  • G Guyonnet
    Department of Ophthalmology, University of Burgundy, Dijon, France.
  • E H Chelqi
    Department of Ophthalmology, University of Burgundy, Dijon, France.
  • L Rochette
    Department of Ophthalmology, University of Burgundy, Dijon, France.
Investigative Ophthalmology & Visual Science May 1995, Vol.36, 1084-1087. doi:
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      A M Bron, V Maupoil, C Garcher, G Guyonnet, E H Chelqi, L Rochette; Modification of vitamin E during ischemia-reperfusion in rat retina.. Invest. Ophthalmol. Vis. Sci. 1995;36(6):1084-1087.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine the variations of vitamin E in rat retinas after transient ischemia and reperfusion. METHODS: Male Wistar rats 11 to 12 weeks of age were used in all experiments. Ischemia was induced by ligation of the optic nerve and vessels, then reperfusion was obtained by removing the ligature. Retinas were dissected, and vitamin E was analyzed by a modified high-performance liquid chromatography assay. RESULTS: There was an insignificant rise in vitamin E during ischemia and a dramatic decrease after reperfusion. No changes were dependent on the period of ischemia (30, 60, or 90 minutes) or reperfusion (15 or 120 minutes). CONCLUSION: Vitamin E decreased only in the reperfused groups, demonstrating a depletion of defenses against free radical production occurring during reperfusion. Vitamin E changes may represent an indirect measure of lipoperoxidation during ischemia-reperfusion and, thus, a potential parameter to evaluate drugs in vitro.

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