October 1995
Volume 36, Issue 11
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Articles  |   October 1995
Dystrophin localization at presynapse in rat retina revealed by immunoelectron microscopy.
Author Affiliations
  • H Ueda
    Department of Anatomy, Yamanashi Medical University, Japan.
  • T Kobayashi
    Department of Anatomy, Yamanashi Medical University, Japan.
  • K Mitsui
    Department of Anatomy, Yamanashi Medical University, Japan.
  • K Tsurugi
    Department of Anatomy, Yamanashi Medical University, Japan.
  • S Tsukahara
    Department of Anatomy, Yamanashi Medical University, Japan.
  • S Ohno
    Department of Anatomy, Yamanashi Medical University, Japan.
Investigative Ophthalmology & Visual Science October 1995, Vol.36, 2318-2322. doi:
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      H Ueda, T Kobayashi, K Mitsui, K Tsurugi, S Tsukahara, S Ohno; Dystrophin localization at presynapse in rat retina revealed by immunoelectron microscopy.. Invest. Ophthalmol. Vis. Sci. 1995;36(11):2318-2322.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: It has been accepted that retinal dystrophin is localized in the outer plexiform layer (OPL) of the retina, but no electron microscopic evidence has been reported until now. Using immunoelectron microscopy, the authors investigate whether retinal dystrophin localizes at the presynaptic or postsynaptic membrane of synaptic regions. METHODS: Monoclonal antibody raised against human dystrophin C-terminus was used. Immunoblotting analysis was used for testing dystrophin protein in retinal tissues. Tissue preparations were stained with the immunofluorescence or immunoperoxidase method and were observed with light and electron microscopy. RESULTS: Immunoblotting analysis showed a molecular weight of band 420 kDa in the sodium dodecyl sulfate--polyacrylamide gel electrophoresis of the retinal tissues. Immunoelectron microscopy disclosed that retinal dystrophin was localized at the presynaptic membrane of synaptic regions in the OPL. CONCLUSIONS: Rat retinal dystrophin localizes at the presynaptic membrane in the OPL, suggesting that it may play some role in the neurotransmitter release of the photoreceptor cell.

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