September 1995
Volume 36, Issue 10
Free
Articles  |   September 1995
A new model of subretinal neovascularization in the rabbit.
Author Affiliations
  • H Kimura
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • T Sakamoto
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • D R Hinton
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • C Spee
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • Y Ogura
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • Y Tabata
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • Y Ikada
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
  • S J Ryan
    Doheny Eye Institute, Los Angeles, CA 90033, USA.
Investigative Ophthalmology & Visual Science September 1995, Vol.36, 2110-2119. doi:
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      H Kimura, T Sakamoto, D R Hinton, C Spee, Y Ogura, Y Tabata, Y Ikada, S J Ryan; A new model of subretinal neovascularization in the rabbit.. Invest. Ophthalmol. Vis. Sci. 1995;36(10):2110-2119.

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Abstract

PURPOSE: To establish a new model of subretinal neovascularization (SRN) in the rabbit by implanting basic fibroblast growth factor (bFGF)-impregnated gelatin microspheres beneath the retina. METHODS: Basic fibroblast growth factor-impregnated gelatin microspheres were prepared by forming a polyion complex between gelatin and bFGF. The microspheres, containing 2.5 micrograms of bFGF, were injected into the subretinal space of rabbit eyes (n = 29). Control eyes (n = 10) received bFGF-free gelatin microspheres. Eyes were followed up for 3 days to 8 weeks by ophthalmoscopy, photography, fluorescein angiography, light microscopy, and transmission electron microscopy. RESULTS: Twenty of 24 experimental eyes (83%) showed fluorescein leakage from SRN 2 weeks after implantation of the bFGF-impregnated microspheres. This leakage continued for 2 to 6 more weeks. In striking contrast, control eyes showed no fluorescein leakage. Histologic examination revealed SRN in all the experimental eyes but in none of the control eyes. CONCLUSIONS: Subretinal implantation of bFGF-impregnated gelatin microspheres induces reproducible SRN in the rabbit.

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