April 1995
Volume 36, Issue 5
Free
Articles  |   April 1995
Melanogenesis by human uveal melanocytes in vitro.
Author Affiliations
  • D N Hu
    Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
  • S A McCormick
    Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
  • S J Orlow
    Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
  • S Rosemblat
    Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
  • A Y Lin
    Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
  • K Wo
    Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
Investigative Ophthalmology & Visual Science April 1995, Vol.36, 931-938. doi:
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    • Get Citation

      D N Hu, S A McCormick, S J Orlow, S Rosemblat, A Y Lin, K Wo; Melanogenesis by human uveal melanocytes in vitro.. Invest. Ophthalmol. Vis. Sci. 1995;36(5):931-938.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To study melanogenesis by cultured human uveal melanocytes, and the relationship between melanin production by uveal melanocytes in vitro with the degree of iris pigmentation in vivo. METHODS: Melanin content, melanin production, and tyrosinase activity of cultured uveal melanocytes derived from eyes of various iris color were measured at different stages of cultivation. RESULTS: Cultured uveal melanocytes maintained a constant level of melanin content, expressed tyrosinase activity, and produced measurable amounts of melanin in vitro. Melanosomes in different stages were seen ultrastructurally. Melanin production correlated directly with the degree of iris pigmentation of the eyes from which the uveal melanocytes were isolated. Tyrosinase activity of cultured uveal melanocytes from black versus white donors was significantly different, but, among white donors, there was no correlation with iris pigmentation or with melanin production in vitro. CONCLUSION: Cultured uveal melanocytes can produce melanin in vitro. Cultured uveal melanocytes isolated from eyes of different iris color maintained their inherent capacity for melanogenesis. Therefore, cultured uveal melanocytes are an excellent model system for studying melanogenesis in uveal melanocytes in vitro.

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