September 1995
Volume 36, Issue 10
Free
Articles  |   September 1995
Functional Rescue of photoreceptors from the damaging effects of constant light by survival-promoting factors in the rat.
Author Affiliations
  • K Masuda
    Department of Ophthalmology, Hamamatsu University, School of Medicine, Japan.
  • I Watanabe
    Department of Ophthalmology, Hamamatsu University, School of Medicine, Japan.
  • K Unoki
    Department of Ophthalmology, Hamamatsu University, School of Medicine, Japan.
  • N Ohba
    Department of Ophthalmology, Hamamatsu University, School of Medicine, Japan.
  • T Muramatsu
    Department of Ophthalmology, Hamamatsu University, School of Medicine, Japan.
Investigative Ophthalmology & Visual Science September 1995, Vol.36, 2142-2146. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K Masuda, I Watanabe, K Unoki, N Ohba, T Muramatsu; Functional Rescue of photoreceptors from the damaging effects of constant light by survival-promoting factors in the rat.. Invest. Ophthalmol. Vis. Sci. 1995;36(10):2142-2146.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To investigate whether and how survival-promoting agents rescue photoreceptor cell function and morphology from constant light damage, the authors recorded electroretinographic (ERG) responses and examined light micrographs of retinas in those rats given intravitreal injection of midkine (MK) and basic fibroblast growth factor (bFGF) before constant exposure. METHODS: Albino Sprague-Dawley rats were injected with MK, bFGF, or phosphate-buffered saline (PBS) 2 days before the onset of 1 week of constant light. ERG responses were recorded using white flash stimuli with the intensity range of 4 log units, followed by histologic examinations of retinas, including quantitative assessment of the outer nuclear layer thickness as an index of photoreceptor cell loss. RESULTS: ERG responses were barely detectable in uninjected eyes after 1 week of constant light. On the other hand, distinct responses were recordable in eyes injected intravitreally with MK and bFGF, and the degree of ERG rescue in terms of the amplitude of b-wave was approximately 40% to 60% compared with normal eyes. Intravitreally injected PBS showed slight, but noticeable, preservation of ERG responses as well. Histologic examination revealed that MK and bFGF protected photoreceptors from light damage. A good correlation was found between anatomic rescue of photoreceptors as assessed by outer nuclear layer thickness and the functional rescue as defined by the magnitude of ERG responses. CONCLUSIONS: Functional and anatomic rescue of photoreceptors in albino rats from constant light damage is achieved by prior intravitreal injection of MK and bFGF.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×