September 1995
Volume 36, Issue 10
Free
Articles  |   September 1995
Detection of mRNA for the cytokines, interleukin-1 alpha and interleukin-8, in corneas from patients with pseudophakic bullous keratopathy.
Author Affiliations
  • J T Rosenbaum
    Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201, USA.
  • S T Planck
    Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201, USA.
  • X N Huang
    Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201, USA.
  • L Rich
    Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201, USA.
  • J C Ansel
    Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201, USA.
Investigative Ophthalmology & Visual Science September 1995, Vol.36, 2151-2155. doi:
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      J T Rosenbaum, S T Planck, X N Huang, L Rich, J C Ansel; Detection of mRNA for the cytokines, interleukin-1 alpha and interleukin-8, in corneas from patients with pseudophakic bullous keratopathy.. Invest. Ophthalmol. Vis. Sci. 1995;36(10):2151-2155.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: Cytokines have been implicated in corneal inflammatory diseases. The authors sought to determine if corneal diseases with different patterns of inflammation are accompanied by characteristic patterns of cytokine expression. METHODS: The authors used reverse transcription-polymerase chain reaction to detect mRNA for 12 specific cytokines from patients with Fuch's dystrophy (n = 7), pseudophakic bullous keratopathy (n = 12), or normal corneas (n = 4). RESULTS: Using Wilcoxon rank sum analysis, mRNA for interleukin (IL)-1 alpha or IL-8 was significantly more abundant in corneas from patients with pseudophakic bullous keratopathy relative to either comparison group. mRNA for the T-cell marker, CD4, and for T-cell derived cytokines, IL-2, IL-4, and interferon gamma, could not be detected in any corneal sample. Message for IL-1 receptor antagonist and transforming growth factor-beta 1 or -beta 2 was readily detectable in most corneas regardless of diagnosis. CONCLUSION: The findings indicate that the pattern of cytokine mRNA expression reflects differences in the pathogenesis of these corneal diseases.

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