March 1994
Volume 35, Issue 3
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Articles  |   March 1994
Cytokines in vitreous humor: interleukin-6 is elevated in proliferative vitreoretinopathy.
Author Affiliations
  • D J Kauffmann
    Department of Ophthalmology, New York Medical College, Valhalla.
  • J C van Meurs
    Department of Ophthalmology, New York Medical College, Valhalla.
  • D A Mertens
    Department of Ophthalmology, New York Medical College, Valhalla.
  • E Peperkamp
    Department of Ophthalmology, New York Medical College, Valhalla.
  • C Master
    Department of Ophthalmology, New York Medical College, Valhalla.
  • M E Gerritsen
    Department of Ophthalmology, New York Medical College, Valhalla.
Investigative Ophthalmology & Visual Science March 1994, Vol.35, 900-906. doi:
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      D J Kauffmann, J C van Meurs, D A Mertens, E Peperkamp, C Master, M E Gerritsen; Cytokines in vitreous humor: interleukin-6 is elevated in proliferative vitreoretinopathy.. Invest. Ophthalmol. Vis. Sci. 1994;35(3):900-906.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

PURPOSE: To measure the levels of interleukins (IL) 1 beta, 6, and 8, and tumor necrosis factor-alpha (TNF alpha) in the vitreous of patients with proliferative vitreoretinopathy (PVR), proliferative diabetic retinopathy (PDR), vitreous hemorrhage, and macular pucker. METHODS: Vitreous samples were collected, undiluted, from patients with PVR, PDR of varying severity, and miscellaneous lesions (vitreous hemorrhage from trauma, macular degeneration, vein occlusion, and non-PVR patients with giant tear, retinal detachment, and macular pucker). Immunoreactive levels of the cytokines, IL-1 beta, IL-6, IL-8, and TNF alpha were determined by enzyme-linked immunoadsorbent assays, and samples were analyzed for protein and hyaluronic acid content using standard assays. RESULTS: The levels of TNF alpha were below detection limits of the assay (< 3 pg/ml). In 45 of the 47 samples tested, IL-1 beta levels also were below detection limits of the assay (< 3 pg/ml). IL-6 levels ranged from < 30 to 5487 pg/ml, with the highest values observed in the PVR patients. IL-8 levels ranged from < 20 to 1900 pg/ml, and were consistently high in the miscellaneous group. Some of the PVR patients with C2 and C3 level severity also exhibited IL-8 levels exceeding 100 pg/ml. In a second study, IL-6 content of vitreous from miscellaneous and PVR patients was compared. In this study, significantly elevated levels of IL-6 were observed in the PVR patients (91.5 +/- 18 pg/ml) compared to the miscellaneous group (10.3 +/- 3.7 pg/ml) CONCLUSIONS: Elevated levels of IL-6 in the vitreous occur in PVR, implicating a role for this cytokine in the pathogenesis of this ocular disorder.

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