July 1995
Volume 36, Issue 8
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Articles  |   July 1995
Photocoagulated human retinal pigment epithelial cells produce an inhibitor of vascular endothelial cell proliferation.
Author Affiliations
  • N Yoshimura
    Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.
  • M Matsumoto
    Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.
  • H Shimizu
    Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.
  • M Mandai
    Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.
  • Y Hata
    Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.
  • T Ishibashi
    Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.
Investigative Ophthalmology & Visual Science July 1995, Vol.36, 1686-1691. doi:
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    • Get Citation

      N Yoshimura, M Matsumoto, H Shimizu, M Mandai, Y Hata, T Ishibashi; Photocoagulated human retinal pigment epithelial cells produce an inhibitor of vascular endothelial cell proliferation.. Invest. Ophthalmol. Vis. Sci. 1995;36(8):1686-1691.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine if conditioned culture medium of photocoagulated human retinal pigment epithelial (RPE) cells contains inhibitors for the proliferation of bovine aortic endothelial cells (BAEC) and bovine retinal endothelial cells (BREC) and to characterize the nature of these inhibitory factors. METHODS: Retinal pigment epithelial cells grown to confluence were photocoagulated (0.1 second, 50 microns, 350 mW) in serum-free medium. After 48 hours, the conditioned medium (PC-CM) was removed, and the effects of non-acid-treated and transiently acid-treated samples were determined on [3H]-thymidine uptake by BAEC and BREC. PC-CM was also subjected to size exclusion high-performance liquid chromatography (HPLC). Fractions were analyzed for the effects on the growth of the bovine endothelial cells before and after transient acid treatment. RESULTS: The addition of non-acid-treated PC-CM (32% vol/vol) inhibited BREC [3H]-thymidine uptake to 18.5% of the control value. With HPLC, the inhibitory activity was recovered mainly in a fraction whose apparent molecular size was 25 kd. After transient acid treatment of the fractions, there also appeared a 100-kd inhibitor. Inhibitory effects were neutralized by pretreatment of the fractions with antiserum against transforming growth factor (TGF)-beta 2. CONCLUSIONS: Photocoagulated RPE cells secrete inhibitors of BAEC and BREC proliferation. Molecular size and immunologic properties of these inhibitors correspond to those of TGF-beta 2.

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