January 1997
Volume 38, Issue 1
Free
Articles  |   January 1997
Effect of transforming growth factor beta-1 in endotoxin-induced uveitis.
Author Affiliations
  • B Peng
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • Q Li
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • F G Roberge
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • C C Chan
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
Investigative Ophthalmology & Visual Science January 1997, Vol.38, 257-260. doi:
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    • Get Citation

      B Peng, Q Li, F G Roberge, C C Chan; Effect of transforming growth factor beta-1 in endotoxin-induced uveitis.. Invest. Ophthalmol. Vis. Sci. 1997;38(1):257-260.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: Transforming growth factor beta-1 (TGF beta-1) can modulate inflammation. Endotoxin-induced uveitis (EIU) is characterized by acute ocular inflammation related to the release of cytokines, including interleukin (IL)-6. The authors investigated the effect of TGF beta-1 on EIU in mice. METHODS: Three independent experiments were performed. Endotoxin-induced uveitis was induced in C3H/HeN mice by an injection of 200 micrograms of lipopolysaccharide (LPS). Two micrograms of TGF beta-1 in 0.1 ml phosphate-buffered saline (PBS) or 0.1 ml PBS alone was administered intraperitoneally at 8 hours after LPS injection. Twenty-four hours after LPS injection, the aqueous humor of the right eyes was collected for leukocyte count, protein concentration, and IL-6 assay. Left eyes were processed for routine histology. RESULTS: TGF beta-1-treated mice showed less ocular inflammation histologically than to the animals that were given PBS. This was confirmed by decreases in leukocyte count, protein concentration, and IL-6 level in the aqueous humor. CONCLUSIONS: TGF beta-1 inhibits the development of EIU. TGF beta-1 may be useful for the modulation of uveitis in humans.

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