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Masanori Ino-ue, Lixin Zhang, Hiroaki Naka, Hiroshi Kuriyama, Misao Yamamoto; Polyol Metabolism of Retrograde Axonal Transport in Diabetic Rat Large Optic Nerve Fiber. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4055-4058.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. The role of the polyol pathway metabolism in progressive impairment of
retrograde axonal transport was evaluated in the optic nerve of rats
with streptozotocin-induced diabetes.
methods. Rats with streptozotocin-induced diabetes received a low (3 mg/kg body
weight) or high dose (10 mg/kg body weight) of oral aldose reductase
inhibitor (ARI). At 1 and 3 months after induction of diabetes,
Fluoro-Gold (FG, Chemicon, Temecula, CA) was injected into the dorsal
lateral geniculate nucleus. Percentages of FG–labeled large, medium,
and small retinal ganglion cells (RGCs) per total population were
calculated in the retinas of ARI-treated diabetic, untreated diabetic,
and normal control rats.
results. Mean percentages of FG–labeled large RGCs per total population were
significantly decreased in nontreated diabetic rats compared with
control animals at 1 month of induced diabetes. This decrease in FG
labeling was not observed in both the low- and high-dose ARI-treated
diabetic rats. At 3 months of induced diabetes, FG labeling of both
large and medium RGCs was significantly decreased. This decrease was
completely ameliorated by high-dose ARI treatment.
conclusions. These results indicate that diabetes affects retrograde axonal
transport progressively through selective impairment of RGCs and that
the polyol pathway metabolism is involved in such
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