Purchase this article with an account.
Esen Karamursel Akpek, Sammy H. Liu, John D. Gottsch; Induction of Experimental Autoimmune Keratitis by Adoptive Transfer of Human Corneal Antigen–Specific T-Cell Line. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4182-4188.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To establish a permanent human corneal antigen (HuCOAg)-specific T-cell
line and to determine whether line cells are capable of inducing
inflammatory keratitis by adoptive transfer.
methods. Lymphoid cells harvested from HuCOAg-immunized Lewis rats were expanded
to a permanent T-cell line by repetitive cycles of restimulation with
HuCOAg and irradiated antigen-presenting cells and propagation in
interleukin 2–containing medium. The phenotype and epitope specificity
of the line cells were determined. Adoptive transfer was performed
after seven cycles by intraperitoneal injection of activated T cells
into irradiated recipient rats.
results. A panel of 11 overlapping synthetic HuCOAg peptides to identify T-cell
epitopes recognized by the line cells was used. The cells responded
selectively to a synthetic peptide containing an immunodominant epitope
of HuCOAg (peptides 69–83). Line cells bore the surface phenotype of
the T-helper/inducer marker (W 3/25+ or CD4+).
Intraperitoneal inoculation of naive rats with 5 ×
107 activated line cells led to maximal clinical signs of
stromal keratitis 7 to 9 days after transfer, characterized by corneal
haze, conjunctival and episcleral injection, corneal infiltrates, and
neovascularization. Histopathologic examination of the tissues revealed
numerous lymphocytes and macrophages and some polymorphonuclear
leukocytes along with neovascularization. The pathologic lesions were
confined to the peripheral corneal stroma. Immunohistochemical studies
demonstrated that the overwhelming majority of the inflammatory cells
were CD4+ T lymphocytes and macrophages; an upregulation of
major histocompatibility complex class II antigen expression was also
conclusions. A long-term, rat T-cell line of CD4+ phenotype specific for
HuCOAg that can induce autoimmune keratitis by adoptive transfer of the
line cells to naive syngeneic recipients is described. With the
development of this cell line, the mechanisms by which T cells exert
their immunopathologic effects in experimental autoimmune keratitis
models can be studied.
This PDF is available to Subscribers Only