Purchase this article with an account.
Hong–Kit Poon, Mark O. M. Tso, Tim T. Lam; c-Fos Protein in Photoreceptor Cell Death after Photic Injury in Rats. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2755-2758.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To examine the involvement of c-Fos protein in light-induced
photoreceptor cell death in rats.
methods. Thirty-two Lewis albino rats were exposed to green fluorescent light
(480–520 nm) of 300 to 320 foot-candles (3228–3443.2 lux) for 3
hours, allowed to recover in the dark, and euthanatized at 0, 1, 3, 6,
12, 24, or 96 hours after light exposure. c-Fos was detected
immunohistochemically and nicked DNA by in situ TdT-dUTP terminal
nick-end labeling (TUNEL). Double labeling of c-Fos and DNA nicks was
results. There was a time-dependent change in the number of c-Fos–positive
photoreceptor nuclei after light injury, which paralleled the change in
the number of TUNEL-positive nuclei. The temporal and spatial
appearance of these nuclei also matched the appearance of pyknotic
nuclei of the outer nuclear layer. Double-labeling study revealed that
some c-Fos–positive nuclei were also TUNEL-positive nuclei.
conclusions. There was an acute accumulation of c-Fos protein in photoreceptors
associated with cell death. This study further supports other studies
showing that c-Fos is linked to apoptotic photoreceptor cell
This PDF is available to Subscribers Only