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Atsushi Nonaka, Junichi Kiryu, Akitaka Tsujikawa, Kenji Yamashiro, Kazuaki Miyamoto, Hirokazu Nishiwaki, Michiko Mandai, Yoshihito Honda, Yuichiro Ogura; Administration of 17β-Estradiol Attenuates Retinal Ischemia–Reperfusion Injury in Rats. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2689-2696.
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purpose. Accumulating evidence has suggested that 17β-estradiol exerts
protective effects against ischemic damage in various organs. In
addition, leukocytes that accumulate in postischemic tissues are
thought to play a central role in ischemia–reperfusion injury. This
study was designed to evaluate quantitatively the inhibitory effects of
17β-estradiol on leukocyte accumulation during ischemia–reperfusion
injury and on subsequent retinal damage after transient retinal
methods. Transient (60 minutes) retinal ischemia was induced in male rats by
temporary ligation of the optic nerve. Thirty minutes before induction
of ischemia, 17β-estradiol (0.1 mg/kg) was administered
intraperitoneally. At 6, 12, 24, and 48 hours after reperfusion,
leukocyte accumulation in the retina was evaluated in vivo by means of
acridine orange digital fluorography. Histologic and
electroretinographic (ERG) studies were carried out to evaluate retinal
results. Treatment with 17β-estradiol significantly inhibited postischemic
leukocyte accumulation; the maximum number of accumulating leukocytes
was reduced by 35.7% at 24 hours after reperfusion (P = 0.01). Histologic examination showed that administration of
17β-estradiol significantly reduced retinal damage, which was most
obvious in the inner retina, 168 hours after reperfusion
(P = 0.0001). ERG studies at 12 and 168 hours after
reperfusion showed that recovery of the b-wave amplitude was
significantly improved with treatment of 17β-estradiol
(P = 0.023).
conclusions. The present study demonstrated the inhibitory effects of
17β-estradiol on leukocyte accumulation and subsequent tissue injury
during retinal ischemia–reperfusion injury.
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