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Klaus Ruether, Johannes Grosse, Eike Matthiessen, Kirstin Hoffmann, Christian Hartmann; Abnormalities of the Photoreceptor-Bipolar Cell Synapse in a Substrain of C57BL/10 Mice. Invest. Ophthalmol. Vis. Sci. 2000;41(12):4039-4047.
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purpose. A preferential loss of ERG b-wave was detected in a substrain of
C57BL/10 mice. Electroretinographic and histologic techniques were used
to investigate this hitherto unknown retinopathy.
methods. ERGs were obtained from normal and affected C57BL/10. C57BL/6 mice
served also as controls. a-Wave and c-wave analyses were performed.
Microscopic investigations were done at two different ages.
results. In the scotopic ERG, a severe reduction of the b-wave amplitude could
be observed, whereas the a-wave was only moderately attenuated
(“negative ERG”). With age, the a-wave amplitude further decreased,
but the rate of reduction was comparable to normals. Oscillatory
potentials were severely altered, and the photopic ERG was
absent. The ERG c-wave was comparable to normal. ERG a-wave
analysis also revealed a reduced maximum amplitude, but no significant
difference of receptor sensitivity. Light microscopy revealed a
thinning of all retinal layers but mostly of the outer plexiform layer.
The number of photoreceptor nuclei was reduced by one third. Electron
microscopy revealed a profound loss of ribbon-shaped synapses between
rod and rod-bipolar cells and severely abnormal ribbons in the case of
conclusions. The so-called negative ERG was associated with alterations in the
synaptic layer between rods and rod bipolars. The absent cone ERG may
be due to the altered cone-on-bipolar synapses. The overall thinning of
the retina as well as the moderately reduced scotopic a-wave amplitude
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