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Jiang Li, Rajkumar V. Patil, A. S. Verkman; Mildly Abnormal Retinal Function in Transgenic Mice without Müller Cell Aquaporin-4 Water Channels. Invest. Ophthalmol. Vis. Sci. 2002;43(2):573-579.
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purpose. Immunocytochemistry showed strong aquaporin (AQP)-4 water channel
expression in Müller cells in mouse retina and fibrous astrocytes
in optic nerve. This study was designed to test the hypothesis that
AQP4 is required for vision by comparing electroretinograms and retinal
morphology in wild-type mice and transgenic knockout mice with no AQP4.
methods. Electroretinograms were recorded over a 105-fold range of
flash intensities in dark-adapted mice and analyzed for a- and b-wave
amplitude and latency, a-wave normalized slope, and oscillatory
potential amplitude and latency. AQP4 protein was localized in mouse
retina by immunocytochemistry, and retinal morphology was studied by
light and electron microscopy.
results. Significantly reduced electroretinogram b-wave potentials were recorded
in 10-month-old null mice with smaller changes in 1-month-old mice.
Immunocytochemistry showed strong AQP4 protein expression in retina of
wild-type mice. Morphologic analysis of retina by light and electron
microscopy showed no differences in retinal ultrastructure.
conclusions. Retinal function is mildly impaired in AQP4-null mice, suggesting a
role for AQP4 in Müller cell fluid balance. These results support
the paradigm that AQP4 expression in supportive cells in the nervous
system facilitates neural signal transduction in nearby electrically
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