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Francine F. Behar–Cohen, Brigitte Thillaye–Goldenberg, Thérèse de Bizemont, Michelle Savoldelli, Dominique Chauvaud, Yvonne de Kozak; EIU in the Rat Promotes the Potential of Syngeneic Retinal Cells Injected into the Vitreous Cavity to Induce PVR. Invest. Ophthalmol. Vis. Sci. 2000;41(12):3915-3924.
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purpose. To determine whether syngeneic retinal cells injected in the vitreous
cavity of the rat are able to initiate a proliferative process and
whether the ocular inflammation induced in rats by lipopolysaccharide
(LPS) promotes this proliferative vitreoretinopathy (PVR).
methods. Primary cultured differentiated retinal Müller glial (RMG) and
retinal pigmented epithelial (RPE) cells isolated from 8 to 12
postnatal Lewis rats were injected into the vitreous cavity of 8- to
10-week-old Lewis rats (105 cells/eye in 2 μl sterile
saline), with or without the systemic injection of 150 μg LPS to
cause endotoxin-induced uveitis (EIU). Control groups received an
intravitreal injection of 2 μl saline. At 5, 15, and 28 days after
cell injections, PVR was clinically quantified, and
immunohistochemistry for OX42, ED1, vimentin (VIM), glial fibrillary
acidic protein (GFAP), and cytokeratin was performed.
results. The injection of RMG cells, alone or in combination with RPE cells,
induced the preretinal proliferation of a GFAP-positive tissue, that
was enhanced by the systemic injection of LPS. Indeed, when EIU was
induced at the time of RMG cell injection into the vitreous cavity, the
proliferation led to retinal folds and localized tractional
detachments. In contrast, PVR enhanced the infiltration of inflammatory
cells in the anterior segment of the eye.
conclusions. In the rat, syngeneic retinal cells of glial origin induce PVR that is
enhanced by the coinduction of EIU. In return, vitreoretinal glial
proliferation enhanced the intensity and duration of
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