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Fumiyuki Uehara, Norio Ohba, Masayuki Ozawa; Isolation and Characterization of Mucinlike Glycoprotein Associated with Photoreceptor Cells. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2759-2765.
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purpose. Although previous lectin-histochemical studies have shown that O-linked
glycoproteins are distributed in cone pedicles and rod spherules, as
well as in photoreceptors, including associated interphotoreceptor
matrices (IPM), attention has been directed only to those in the IPM.
In this study, cloning of the O-linked glycoproteins not only in the
IPM but also in the region including the cone pedicles and rod
spherules was attempted.
methods. The cDNA for the core protein of the O-linked glycoprotein in the
bovine retina was isolated by screening a bovine retinal cDNA library
using a polyclonal antibody against the jacalin (a lectin specific for
O-linked sugar residues)-binding glycoproteins (JBGPs) in the whole
bovine retina. The expression of the JPGP core protein in the retina
was examined by means of in situ hybridization histochemistry and
results. The cDNA was isolated and found to encode an entire core protein[
predicted molecular mass (Mr): 101 kDa; rich in Ser and
Thr; mucin-like] for the JBGPs with Mr of 120 and 135 kDa.
The mRNA was expressed in both cone and rod photoreceptor cells. This
protein was distributed in the cone pedicles and rod spherules as well
as the photoreceptor layer.
conclusions. Mucinlike glycoproteins with Mr of 120 and 135 kDa may be
synthesized in the cone and rod photoreceptor cells, respectively, and
distributed not only in the photoreceptor layer (probably including the
IPM) but also in the cone pedicles and rod
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