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Masaru Inatani, Hidenobu Tanihara, Atsuhiko Oohira, Megumi Honjo, Noriaki Kido, Yoshihito Honda; Upregulated Expression of Neurocan, a Nervous Tissue Specific Proteoglycan, in Transient Retinal Ischemia. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2748-2754.
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purpose. Neurocan, a nervous tissue–specific chondroitin sulfate proteoglycan
synthesized primarily by neurons, is expressed abundantly in developing
rat retina, whereas it is rarely expressed in adult rat retinas. This
study investigated the reexpression of neurocan in a pathologic
condition of adult rat retina.
methods. Transient retinal ischemia was produced by occlusion of the retinal
artery for 60 minutes. After transient retinal ischemia, neurocan
expression was investigated by reverse transcription–initiated
polymerase chain reaction (RT-PCR), immunohistochemistry, and
results. Semiquantitative analysis using RT-PCR revealed that mRNA expression
for neurocan increased at 24 hours after reperfusion. Furthermore, on
immunoblot analysis using an anti-neurocan antibody, MAb 1G2, the
intensity of the 220-kDa band as well as the 150-kDa band increased
markedly at 24 and 72 hours after reperfusion. The 220-kDa band was
predominant at 24 hours after reperfusion, whereas the intensity of the
150-kDa band became almost the same as that of the 220-kDa band at 72
hours after reperfusion. Immunohistochemical analysis revealed that
upregulated neurocan immunoreactivity was associated with glial
conclusions. Thus, upregulated expression of neurocan in transient retinal ischemia
was demonstrated. Furthermore, the immunohistochemical analysis
revealed that the upregulated expression of neurocan is derived from
Müller cells, although it has been thought that neurocan is
synthesized by neurons so far. The neurocan expression by Müller
cells suggests that this proteoglycan plays a role in the damage and
repair processes in diseased retina.
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