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Sotaro Ooto, Masatoshi Haruta, Yoshihito Honda, Hiroshi Kawasaki, Yoshiki Sasai, Masayo Takahashi; Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells. Invest. Ophthalmol. Vis. Sci. 2003;44(6):2689-2693. doi: 10.1167/iovs.02-1168.
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purpose. To produce lens cells from primate embryonic stem (ES) cells in a reproducible, controlled manner.
methods. Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. The effect of varying the concentration of fibroblast growth factor (FGF)-2 and the density of the ES colonies plated during the differentiation process were also examined.
results. After a 2- to 3-week induction period, lentoids were produced by a subpopulation of ES colonies. Western blot analysis and immunohistochemistry revealed that these lentoids expressed αA-crystallin and Pax6. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density.
conclusions. The differentiation of primate ES cells into lentoids can be achieved by treatment with SIDA. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro.
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