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Oliver Rinner, Jens M. Rick, Stephan C. F. Neuhauss; Contrast Sensitivity, Spatial and Temporal Tuning of the Larval Zebrafish Optokinetic Response. Invest. Ophthalmol. Vis. Sci. 2005;46(1):137-142. doi: https://doi.org/10.1167/iovs.04-0682.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To characterize the quantitative properties of the optokinetic response (OKR) in zebrafish larvae as a tool to test visual performance in genetically modified larvae.
methods. Horizontal OKR was triggered in 5-day-old zebrafish larvae by stimulation with projected computer-generated gratings of varying contrast, angular velocity, temporal and spatial frequency, and brightness. Eye movements were analyzed by a custom-made eye tracker based on image analysis.
results. The gain of the OKR slow phase was dependent on angular velocity, spatial frequency, and contrast of a moving grating, but largely independent on brightness. Eye velocity was a logarithmically linear function of grating contrast with a slope of approximately 0.8 per log unit contrast.
conclusions. The OKR of the larval zebrafish is not scaled for stimulus contrast and spatial frequency. These properties make the OKR a valuable tool to quantify behavioral visual performance such as visual acuity, contrast sensitivity, and light adaptation. This behavioral paradigm will be useful for analyzing visual performance in mutant and gene-knockdown larval zebrafish.
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