August 1968
Volume 7, Issue 4
Free
Articles  |   August 1968
Studies on Lens Protein Polypeptides
Author Affiliations
  • ARNOLD L. SHAPIRO
    Department of Ophthalmology, New York University School of Medicine, New York, N.Y. 10016
Investigative Ophthalmology & Visual Science August 1968, Vol.7, 462-467. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      ARNOLD L. SHAPIRO; Studies on Lens Protein Polypeptides. Invest. Ophthalmol. Vis. Sci. 1968;7(4):462-467.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Methods for the separation and identification of the polypeptide chains of each lens crystallin are basic to the study of these proteins on polyribosomes. Rabbit lens proteins have been deaggregated, denatured, and reduced to polypeptide chains, and separated by polyacrylamide gel electrophoresis. Approximate molecular weights of the resultant chains have been calculated by the same system. Vigorous denaturation and reduction with sodium dodecyl sulfate and 2-mercaptoethanol disrupts all structure other than primary structure, degrading each protein to its component polypeptides. The separation method employed is almost exclusively dependent on molecular size. Deaggregated alpha crystallin is a group of chains, each of about 20,000 molecular weight. The beta crystallin region of the DEAE chromatogram yields three polypeptides of estimated molecular weights 21,000, 23,000, and 29,000. Preliminary studies on the four beta crystallin peaks isolated by DEAE-chromatography suggest that one or more may be composed of aggregates of different polypeptide chains. Gamma crystallins appear to be single polypeptide chain proteins of molecular weights from 18,000 to 21,000. Experiments performed on lens epithelium indicate that a significant percentage of their newly synthesized proteins are nuclear and cytoplasmic proteins other than the crystallins.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×